Comparison of fecal coliform enumeration methods for field analysis in resource limited regions

Volume 2, Issue 2, April 2017     |     PP. 53-66      |     PDF (491 K)    |     Pub. Date: June 19, 2017
DOI:    271 Downloads     7583 Views  

Author(s)

Elmira Nazar, M.Sc. Environmental Science, School of Civil Engineering and Environmental Science, University of Oklahoma, 202 W. Boyd St. Norman, OK 73019
Keith A. Strevett* Ph.D., Professor of Civil and Environmental Engineering, School of Civil Engineering and Environmental Science, University of Oklahoma, 202 W. Boyd St. Norman, OK 73019

Abstract
Fecal enumeration is a key factor in determining the quality of a water source. Accuracy and reliability of fecal analysis is dependent upon several variables, viz. incubation temperature, specific conductivity, pH, nutrient availability, population density, suspended solids concentration, etc. The goal of this study was to determine if a correlation exists between the three common field water testing methods (Easy-Gel method, MilliporeTM Membrane Filtration method and the 3MTM Petrifilm method) of fecal coliform enumeration and temperature fluctuation or population density. Field test methods were examined using varying incubation temperatures and population densities. Results of fecal enumeration from each of the field test methods were compared to a standard test method (MF-8074) under standard operating conditions. Regression correlations for each of the field test methods were establish to determine method reliability under non-ideal conditions. Study results indicated that temperature fluxuations and population density did adversely affect the accuracy of fecal enumeration on all field test methods.

Keywords
drinking water, emerging regions, fecal coliform, surface water, water quality testing

Cite this paper
Elmira Nazar, Keith A. Strevett* Ph.D., Comparison of fecal coliform enumeration methods for field analysis in resource limited regions , SCIREA Journal of Environment. Volume 2, Issue 2, April 2017 | PP. 53-66.

References

[ 1 ] Organization, W.H. and UNICEF., Progress on sanitation and drinking-water. 2013: World Health Organization.
[ 2 ] (USEPA), U.S.E.P.A., Water: monitoring and assessment. . 2013
[ 3 ] An, Y.-J., D.H. Kampbell, and G.P. Breidenbach, Escherichia coli and total coliforms in water and sediments at lake marinas. Environmental Pollution, 2002. 120(3): p. 771-778.
[ 4 ] Rompré, A., et al., Detection and enumeration of coliforms in drinking water: current methods and emerging approaches. Journal of microbiological methods, 2002. 49(1): p. 31-54.
[ 5 ] Trottier, S.S.M.G., Study of four new, field-based, microbiological tests: verification of the hydrogen sulfide (H₂S), Easygel®, Colilert and Petrifilm (tm) tests. 2010, Massachusetts Institute of Technology.
[ 6 ] Dunling, W. and W. FIESSEL, Evaluation of media for simultaneous enumeration of total coliform and Escherichia coli in drinking water supplies by membrane filtration techniques. Journal of Environmental Sciences, 2008. 20(3): p. 273-277.
[ 7 ] Kleinheinz, G.T., et al., Comparison of Petrifilm and Colilert methods for E. coli enumeration in recreational water. Lake and Reservoir Management, 2012. 28(4): p. 328-337.
[ 8 ] O'Keefe, S.F., A field-based study of alternative microbial indicator tests for drinking water quality in Northern Ghana. 2012, Massachusetts Institute of Technology.
[ 9 ] Beloti, V., et al., Evaluation of Petrifilm EC and HS for total coliforms and Escherichia coli enumeration in water. Brazilian Journal of Microbiology, 2003. 34(4): p. 301-304.
[ 10 ] Yáñez, M., C. Valor, and V. Catalán, A simple and cost-effective method for the quantification of total coliforms and Escherichia coli in potable water. Journal of microbiological methods, 2006. 65(3): p. 608-611.
[ 11 ] Julian, T.R., et al., Genotypic and phenotypic characterization of Escherichia coli isolates from feces, hands, and soils in rural Bangladesh via the Colilert Quanti-Tray system. Applied and environmental microbiology, 2015. 81(5): p. 1735-1743.
[ 12 ] Company., H. Coliforms-Fecal: USEPA Membrane Filtration Method. . 2012 [cited 2014; Available from: Retrieved from http://www.hach.com/asset-get.download.jsa?id=7639984036.
[ 13 ] Dufour, A.P., E.R. Strickland, and V.J. Cabelli, Membrane filter method for enumerating Escherichia coli. Applied and environmental microbiology, 1981. 41(5): p. 1152-1158.
[ 14 ] Eaton, A.D., et al., Standard methods for the examination of water and wastewater. 2008, USA: Centennial Edition; ISBN 0-087553-047-8.
[ 15 ] Mendenhall, W.M. and T.L. Sincich, Statistics for Engineering and the Sciences. 2016: CRC Press.
[ 16 ] Stearman, R.L., Statistical concepts in microbiology. Bacteriological reviews, 1955. 19(3): p. 160.
[ 17 ] Aziz, S., M. Jamlos, and M. Jamlos. Escherichia coli detection in different types of water. in Wireless Technology and Applications (ISWTA), 2014 IEEE Symposium on. 2014. IEEE.
[ 18 ] Byappanahalli, M.N., et al., Growth and survival of Escherichia coli and enterococci populations in the macro-alga Cladophora (Chlorophyta). FEMS Microbiology Ecology, 2003. 46(2): p. 203-211.
[ 19 ] Jones, P.G., R.A. VanBogelen, and F.C. Neidhardt, Induction of proteins in response to low temperature in Escherichia coli. Journal of bacteriology, 1987. 169(5): p. 2092-2095.
[ 20 ] Tune, J. and A.C. Elmore. Drinking water field analyses for the detection and Enumeration of Coliform Bacteria in rural Guatemalan Highlands. in World Environmental and Water Resources Congress 2009: Great Rivers. 2009.
[ 21 ] El-Shaarawi, A., S. Esterby, and B. Dutka, Bacterial density in water determined by Poisson or negative binomial distributions. Applied and Environmental Microbiology, 1981. 41(1): p. 107-116.
[ 22 ] Haas, C.N., How to average microbial densities to characterize risk. Water Research, 1996. 30(4): p. 1036-1038.
[ 23 ] Haas, C.N. and B. Heller, Statistics of enumerating total coliforms in water samples by membrane filter procedures. Water Research, 1986. 20(4): p. 525-530.
[ 24 ] Smith, A.R., et al., Enumeration of sublethally injured Escherichia coli O157: H7 ATCC 43895 and Escherichia coli strain B-41560 using selective agar overlays versus commercial methods. Journal of food protection, 2013. 76(4): p. 674-679.
[ 25 ] Vail, J., et al., Enumeration of Waterborne with Petrifilm Plates. Journal of environmental quality, 2003. 32(1): p. 368-373.
[ 26 ] Kim, G., E. Choi, and D. Lee, Diffuse and point pollution impacts on the pathogen indicator organism level in the Geum River, Korea. Science of the Total Environment, 2005. 350(1): p. 94-105.
[ 27 ] Kazi, T., et al., Assessment of water quality of polluted lake using multivariate statistical techniques: A case study. Ecotoxicology and Environmental Safety, 2009. 72(2): p. 301-309.
[ 28 ] Simeonov, V., et al., Assessment of the surface water quality in Northern Greece. Water research, 2003. 37(17): p. 4119-4124.