A Comparative Studies of the Activities of Phenoloxidase, Carboxylesterase and Alkaline Phosphatase from Different Geographic Strains of Flacherie-Infected Bombyx mori

Volume 6, Issue 3, June 2021     |     PP. 25-47      |     PDF (607 K)    |     Pub. Date: May 17, 2021
DOI: 10.54647/agriculture21204    106 Downloads     5686 Views  

Author(s)

Wenchu Li, Department of Sericultural Science, College of Animal Science, South China Agricultural University, Guangzhou, China.
Jingyu Zhang, Department of Sericultural Science, College of Animal Science, South China Agricultural University, Guangzhou, China.
Shizhang Feng, Department of Sericultural Science, College of Animal Science, South China Agricultural University, Guangzhou, China.

Abstract
Flacherie diseases of silkworm caused by complex pathogens but mainly Bacillus spp. in springtime. The paper aims to explore interaction between different geographic strains of pathogens and host, and focuses on studying phenoloxidase (PO) and other enzymes activities in hemolymph, midgut and silk glands. PO activity increased in silkworms infected with Bacillus spp. geographic strain of BsE, and BsC. The activities of PO and carboxylesterase (CE) in the midgut of infected silkworms gradually increased in fourth larval instars, while it decreased in fifth larval instars. Alkaline phosphatase (ALKP) activity was present on the inner and outer membranes of the silk gland of diseased silkworms. The outer membrane of the silk gland was dyed with Diaminoben-zidine (DAB), but the inner membrane excluded dye. A mixture of the Nitro-Blue-Tetrazolium/ 5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP) and DAB methods was used to treat the fat body, and both reactions could be detected. Current research into the PO cascade, CE, and ALKP is further reviewed and discussed in detail.

Keywords
flacherie; Bombyx mori; enzymes; silk gland; pathology; insect immunity

Cite this paper
Wenchu Li, Jingyu Zhang, Shizhang Feng, A Comparative Studies of the Activities of Phenoloxidase, Carboxylesterase and Alkaline Phosphatase from Different Geographic Strains of Flacherie-Infected Bombyx mori , SCIREA Journal of Agriculture. Volume 6, Issue 3, June 2021 | PP. 25-47. 10.54647/agriculture21204

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