Optimization of carrageenan extraction from Kappaphycus alvarezii red algae using microwave assisted extraction method with variation of solvent concentration

Volume 8, Issue 2, April 2023     |     PP. 115-128      |     PDF (1479 K)    |     Pub. Date: March 12, 2023
DOI: 10.54647/physics140518    104 Downloads     4915 Views  

Author(s)

Syaharuddin Kasim, Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Hasanuddin University
Subehan, Laboratory of Phytochemistry, Faculty of Pharmacy, Hasanuddin University
Gemini Alam, Laboratory of Phytochemistry, Faculty of Pharmacy, Hasanuddin University
Yusnita Rifai, Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Hasanuddin University
Ismail, Laboratory of Pharmacognosy, Faculty of Pharmacy, Hasanuddin University
Sarce, Laboratory of Pharmacognosy, Faculty of Pharmacy, Hasanuddin University
Rifka Nurul Utami, Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Hasanuddin University

Abstract
Over time, new extraction methods have been developed, one of which is the Microwave Assisted Extraction (MAE) method. The mechanism of action of this method is to use microwave energy to heat the solvents that come into contact with the sample. The problem is whether carrageenan can be extracted from the red algae Kappaphycus alvarezii using the MAE method. For that reason, this research has been carried out with the aim of determining the optimum conditions of carrageenan extraction with the MAE method. The highest carrageenan yield was obtained by extracting with a 3% KOH solution with a ratio of ingredients and solvents of 1:20 and the extraction time of 30 minutes using a 30% power of 68.92%. Chemical identification results indicate carrageenan characterized by formation of fibrous precipitate after addition of 1% methyl blue solution and FT-IR spectrophotometry results indicate that the type of carrageenan obtained is kappa type which is characterized by the similarity of sulfate ester groups identified at wave number 1230- 1265 cm-1; C-O-C (glycosidic bond) at wave number 1033-1010 cm-1; C-O (3,6 anhydrogalactose) at wave number 925 cm-1, C-O-SO3 (D-galactose-4-sulfate) at wave number 844.82 cm-1 with the main functional groups in the carrageenan kappa standard.

Keywords
Carrageenan, Microwave Assisted Extraction, MAE, Kappaphycus alvarezii

Cite this paper
Syaharuddin Kasim, Subehan, Gemini Alam, Yusnita Rifai, Ismail, Sarce, Rifka Nurul Utami, Optimization of carrageenan extraction from Kappaphycus alvarezii red algae using microwave assisted extraction method with variation of solvent concentration , SCIREA Journal of Physics. Volume 8, Issue 2, April 2023 | PP. 115-128. 10.54647/physics140518

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